Western blot analysis from lysates of MT-2 human T cell line and CHO Chinese hamster ovary cell line either mock transfected or transfected with human IL-27 p28/IL-27A. PVDF membrane was probed with 1 µg/mL of Goat Anti-human IL-27 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-46993) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. Specific bands were detected for the IL-27 EBI3/IL-27B at approximately 32 kDa and IL-27 p28/IL-27A at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions.